Friday, September 28, 2012

Ackman pushed for ouster of P&G CEO McDonald in meeting: WSJ

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Thursday, September 27, 2012

Dynamics of DNA packaging helps regulate formation of heart

ScienceDaily (Sep. 27, 2012) ? A new regulator for heart formation has been discovered by studying how embryonic stem cells adjust the packaging of their DNA. This approach to finding genetic regulators, the scientists say, may have the power to provide insight into the development of any tissue in the body -- liver, brain, blood and so on.

A stem cell has the potential to become any type of cell. Once the choice is made, the cell and other stem cells committed to the same fate divide to form organ tissue.

A University of Washington-led research team was particularly interested in how stem cells turn into heart muscle cells to further research on repairing damaged hearts through tissue regeneration. The leaders of the project were Dr. Charles Murry, a cardiac pathologist and stem cell biologist; Dr. Randall Moon, who studies the control of embryonic development, and Dr. John Stamatoyannopoulos, who explores the operating systems of the human genome.

The paper's lead author is Dr. Sharon Paige, a UW MD-PhD student who completed her Ph.D. in Dr. Murry's lab.

The results are published in the Sept. 28 edition of Cell.

Paige, an aspiring pediatric cardiologist, said, "By identifying regulators of cardiac development, this work has the potential to lead to a better understanding of the causes of congenital heart disease, thereby paving the way for therapeutic advances."

Previously UW researchers had examined the signals that prod cells to grow into various kinds of heart tissue. In this case, the researchers entered a relatively unexplored area. They decided to look at the genetic controls behind the transformation of stem cells into heart tissue.

Because stem cells keep their DNA code under wraps until needed, the scientists examined how this packaging is altered over time to permit reading of portions of the code and thereby produce changes in the cell.

DNA is wound up into a structure called chromatin. "DNA can be packaged as tightly closed, neutral or activated," Murry explained. The tightly closed state, he said, is analogous to setting the brakes on a car.

Like a child who clams up when asked, "What will you be when you grow up?" stem cells are protective of the genes that will determine their future cell type, or what scientists call their cell fate.

"We found that stem cells take great care to avoid turning on cell-fate regulating genes at the wrong time," Murry said. "These genes have their brakes on until they are needed." When the time is right, he said, "the brakes come off and the gas goes on."

He explained that the situation is different for genes that regulate cell functions, in contrast to those that regulate cell fate. Genes that control, for example, the production of proteins that allow the cell to contract or to generate electrical signals do not have such a complex braking system. Those genes can be more readily activated.

The researchers pointed out that it was already known that the patterns stem cells follow to modify their DNA packaging distinguished them from progenitor cells -- cells prepared to begin a lineage of a particular type of cell -- and also from cells that already had a working identity, such as blood or muscle cells.

However , the dynamics of the DNA packaging modifications -- how the packaging is programmed to change over time -- and how these dynamics influence which genes are "exposed" and activated to create, for example, heart muscle cells, was poorly understood.

The UW-led research team learned that, as human embryonic stem cells become heart cells, this differentiation is accompanied by distinctive dynamic alterations in DNA packaging. This tell-tale pattern enabled the scientists to distinguish the key regulators of heart development from other genes. The researchers referred to the carefully timed pattern of changes in the DNA wrapping as a "temporal chromatin signature."

Just as a bank robber leaves incriminating evidence in a handwritten note to the teller, the temporal chromatin signature gave the scientists the clues they needed to hunt down new genes that might be responsible for heart formation.

"We found a bunch of them," Murry said. Their system revealed the top candidate to be the homebox gene MEIS2. This gene seemed an unlikely choice because it had no previous record of participating in heart formation. However, when this gene was removed from a new generation of zebra fish, the developing fish embryos had heart tube formation defects and other heart abnormalities.

Murry and other members of the research team think patterns in DNA unwrapping could be broadly applicable to discovering the genes that regulate other aspects of tissue and organ formation beyond only the heart. Such a research approach might help reveal the major developmental decisions that occur inside of cells as an embryo forms and grows. These revelations could provide information useful to spurring stem cells to form specific tissues for organ repair later in life.

Acknowledging the limitations of a lab system in mimicking what happens inside living cells in the early stages of organ formation in humans, Murry said, "The use of the temporal chromatin signature to discover regulatory genes could give us new insights into human development and new tools to control cell fate."

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The above story is reprinted from materials provided by University of Washington, via EurekAlert!, a service of AAAS.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Sharon?L. Paige, Sean Thomas, Cristi?L. Stoick-Cooper, Hao Wang, Lisa Maves, Richard Sandstrom, Lil Pabon, Hans Reinecke, Gabriel Pratt, Gordon Keller, Randall?T. Moon, John Stamatoyannopoulos, Charles?E. Murry. A Temporal Chromatin Signature in Human Embryonic Stem Cells Identifies Regulators of Cardiac Development. Cell, 2012; 151 (1): 221 DOI: 10.1016/j.cell.2012.08.027

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_health/~3/27hegCkUB3w/120927142526.htm

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Great Samples of Clever Web Banners | The Official Wix Blog

Posted by The Wix Team | September 26th 2012

Online advertising can be fun, creative and exciting. Check out these examples for best practices

Online ads and web banners have a great visual potential that usually goes unnoticed. Advertisers often neglect the creative energy when producing web banners, and accordingly, internet users tend to resent those unattractive rectangles that appear on their screens. The good news is that it doesn?t have to be that way. Web banners can be exciting, funny and creative, and, unlike other types of advertising, they can be interactive and playful. We collected examples of really impressive advertising work, all done for the web! These ads are fun to look at at, they won?t antagonize viewers with noises, blings and animations, and you can bet they generate more revenues as well. A win-win situation :)

National Center for Domestic Violence

National Center for Domestic Violence

McDonald's Online Banner Campaign

McDonald?s Online Banner Campaign

Airport Parking web add

Airport Parking

netup-web_design_banner

Net up

Showcase of Clever Web Banners and Adds

Ikea Squeezes 2,800 Products Inside Single Web Banner Ad

Comedy Bar

Comedy Bar

Showcase of Clever Web Banners and Adds

Hand made burger ? Fathers day web banner

Showcase of Clever Web Banners and Adds

Boohoo Trend Banner

Showcase of Clever Web Banners and Adds

BMW X3 Online Campaign ? World?s Longest Banner

Showcase of Clever Web Banners and Adds

BMW X3 Online Campaign ? Edit Your Own Commercial Banner

Showcase of Clever Web Banners and Adds

BMW X3 Online Campaign ? Comparison Banner and Microsite

And have a look at this great video about advertising:

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Tagged with: Creative Online Campaigns . Featured . Online Ads . Online Marketing . Web Banners

Source: http://www.wix.com/blog/2012/09/clever-web-banners-and-ads/

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Wednesday, September 26, 2012

Universal Celebrates Centennial with Its Best-Ever Box Office Tally

The overall box office results might be mired in one of the worst end-of-summer slumps in years, but for at least one studio, 2012 has been a year to remember -- for all the right reasons.

Universal, currently celebrating its 100th anniversary with a yearlong revival of some of its most beloved films, announced today that last weekend's grosses put the studio past the $1.128 billion mark for the year -- the highest tally in its history.

NBCUniversal CEO Steve Burke -- who was already having a pretty good day after getting the news that NBC topped last night's TV ratings -- issued a congratulatory statement, saying, "Our film team has done a great job starting to turn things around and preparing the studio for the future. They have built some great new franchises, reinvigorated some older ones and acquired some very promising intellectual property. We are excited to see them execute on their plan in the years to come."

As the Hollywood Reporter points out, that $1.128 billion largely came courtesy of two films (The Lorax and Ted, which combined for $432 million), but the news is liable to get even better for Universal, as the studio has four more high-profile films on the docket for the year: Pitch Perfect, The Man with the Iron Fists, This Is 40, and Les Miserables.

As impressive as the numbers are for Universal, it's worth noting that the studio's still only in fourth place among all studios on the year -- Warner Bros. remains in first place, with a 15.9% market share and grosses approaching $1.26 billion. Last year's champ, Paramount, is lagging in a distant seventh place.

Source: http://www.rottentomatoes.com/m/1925952/news/1925952/

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Spain prepares more austerity, protesters battle police

MADRID (Reuters) - Protesters clashed with police in Spain's capital on Tuesday as the government prepared a new round of unpopular austerity measures for the 2013 budget to be announced on Thursday.

Thousands gathered in Neptune plaza, a few meters from El Prado museum in central Madrid, where they formed a human chain around parliament, surrounded by barricades, police trucks and more than 1,500 police in riot gear.

Police fired rubber bullets and beat protesters with truncheons, first as protesters were trying to tear down barriers and later to clear the square. The police said at least 22 people had been arrested and at least 32 injured, including four policemen.

As lawmakers started to leave the parliament shortly after 2100 GMT in official cars or by foot, a few hundred people were still demonstrating in front of the building. Most dispersed shortly afterwards.

The protest, promoted over the Internet by different activist groups, was younger and more rowdy than recent marches called by labor unions. Protesters said they were fed up with cuts to public salaries and health and education.

"My annual salary has dropped by 8,000 euros and if it falls much further I won't be able to make ends meet," said Luis Rodriguez, 36, a firefighter who joined the protest. He said he was considering leaving Spain to find a better quality of life.

With this year's budget deficit target looking untenable, the conservative government is now looking at such things as cuts in inflation-linked pensions, taxes on stock transactions, "green taxes" on emissions or eliminating tax breaks.

The 2013 budget is the second conservative Prime Minister Mariano Rajoy has had to pass since he took office in December. Spain must persuade its European partners that it can cut the budget shortfall by more than 60 billion euros by 2014.

Rajoy has already passed spending cuts and tax hikes worth slightly more than that over the next two years, but half-year figures show the 2012 deficit target slipping from view as tax income forecasts will not be hit due to economic contraction.

He said earlier this month the 2013 budget would cut spending further in all areas of government apart from pensions and borrowing costs.

Spain is at the center of the euro zone debt crisis on concerns the government cannot control its finances and those of highly indebted regions, bitten by a second recession since 2009 which has put one in four workers out of a job.

REGIONAL TENSION

On the regional front, Catalonia, which generates about 20 percent of the national output, announced on Tuesday it would hold early elections on November 25 after its call for more tax autonomy was rejected last week by Rajoy.

Political uncertainty in cash-strapped Catalonia, along with an announcement from southern region Andalucia it might seek a 4.9 billion-euro credit line from the central government, will pile more pressure on Madrid to seek European aid.

Rajoy is holding back from applying for help, which would activate a European Central Bank bond-buying programme and bring down Spain's punishing debt premiums.

With the threat of the plan alone reducing 10-year yields by around 2 percentage points, the cautious leader, known for keeping his cards close to his chest, is playing for time.

Rajoy says he is mulling the conditions of a bailout application, but suspicion that he may wait until after regional elections on October 21 pushed short-term yields higher at auction on Tuesday.

The government is also expected on Thursday or Friday to set a fresh timetable for economic reforms, seen as an attempt to pre-empt strict EU-imposed conditions for aid.

"Let us in, we want to evict you," protesters chanted outside parliament. The number of home evictions has soared as thousands of people have defaulted on mortgage loans.

Demonstrators said they were angry that the state has poured funds into crumbling banks while it is cutting social benefits.

"We're protesting against the cuts. I've had to give up my apartment," said Ondina, a 30-year-old fine arts graduate who is without a job. She said she could not survive on unemployment benefit of 260 euros ($340) a month.

RUNNING OUT OF OPTIONS

Half-year deficit data indicate national accounts are already on a slope that will drive Spain into a bailout. The deficit to end-June stands at over 4.3 percent of gross domestic product, including transfers to bailed out banks, making meeting the 6.3 percent target by the end of the year almost impossible.

On Tuesday, the treasury ministry said the central government deficit to end-August had reached 4.77 percent of GDP, already above its year-end target of 4.5 percent of GDP.

"It's going to be difficult keeping the deficit to around 2 percent in the second half, when the first half was closer to 4 percent, especially since traditionally, the second half deficit is higher than the first," said Juan Ignacio Conde-Ruiz, economist at Madrid's Complutense University.

For 2012, the measures aim to reap savings of over 13 billion euros, but economists see the deficit missing the target by almost 1 percentage points, implying further saving needs of up to 10 billion euros for this year alone.

Rajoy has been careful to highlight the importance of next year's deficit target of 4.5 percent of GDP though any shortfall this year will weigh on 2013's accounts. After slashing civil servants' wages, raising value added tax and cutting health and education spending, Rajoy is running out of options.

(Additional reporting by Iciar Reinlein and Borja Gonzalez; writing by Fiona Ortiz and Julien Toyer; editing by Andrew Roche)

Source: http://news.yahoo.com/spain-prepares-more-austerity-protesters-battle-police-003803453--business.html

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Tuesday, September 18, 2012

How To Be A Tattoo Artist, Arts & Entertainment

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    Bangkok Tattoo, a Love Song - a Krom Song

    Bangkok Tattoo, a Love Song - a Krom Song

    Elusive Cambodian group krom have releases Bangkok Tattoo, complete with a video. A haunting homage to lost love, Bangkok Tattoo was recorded at the Cambodian Living Arts studio and written, performed and sung by enigmatic krom front man Christopher Minko. This smoky session was recorded in memory of Minko's wife, Wassana Panmanee-Minko 'Mam' who passed away in late 2010. The video was filmed in Phnom Penh and features a remarkable performance by Cambodia's internationally recognized leading traditional and modern dancer, Belle Chumvan Sodhachivy. The video's superb images perfectly compliment Minko's gravel-laced growl on this krom release. words + music : christopher minko musicians: guitar + vocals - minko; guitar + arrangement- ian white production + video: ian white video featuring: Belle Chumvan Sodhachivy Follow Krom on Facebook: https://www.facebook.com/KromSong And @KromSong on Twitter: https://twitter.com/#!/kromsong Official website: http://www.themekongsessions.com/ My Bangkok Tattoo She?s lookin' at you My Bangkok Tattoo I?ll always love you It?s the original sin Gets under your skin The darkness of an ink bled view My Bangkok Tattoo She?s lookin' at you My Bangkok Tattoo Your love got me through Sanskrit verse on every curve On skin, so fine, (once mine) It was you who soothed my sacred nerve My Bangkok Tattoo My words were too few My Bangkok Tattoo (Oh) what did I do? My Bangkok Tattoo I?m alone without you A tear shed true, you never knew My Bangkok Tattoo She?s lookin' at you My Bangkok Tattoo I?ll always love you My Bangkok Tattoo (Oh) Mam I love you My Bangkok Tattoo She?s lookin? at you
    Ranked 3.81 / 5 | 156 views | 0 comments

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    3D Tattoo Designs - Weird and Beautiful Art

    3D Tattoo Designs - Weird and Beautiful Art

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    Sexy Tattoo Babe, Crazy Guy, & Lingerie Lounge Gossip - 13

    Sexy Tattoo Babe, Crazy Guy, & Lingerie Lounge Gossip - 13

    Watch more videos at http://www.slumberpartytheater.com Slumber Party Theater Presents: "Sexy Tattoo Babe, Crazy Guy, & Lingerie Lounge Gossip - Part 13!!!" The Tattoo Vixen hangs out with Kevin in the Slumber Party Theater Lingerie Lounge! Beware of strong language and strong opinions! Sponsored by Long Pig: The Movie now playing at http://www.longpigmovie.com WINNER Best Underground Film - Indie Fest USA 2008. Nominated for Best Visual Effects, Best Make-Up, and Best Feature Film - Indie Fest USA 2008 http://www.indiefestusa.com
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    Tattoo Vixen Confessions - 13: Hating Child Abuse!

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